Coding

Part:BBa_K1059014:Design

Designed by: Wenjun Wang   Group: iGEM13_OUC-China   (2013-09-16)


RBS B0032+mamK coding squence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 336
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 358
    Illegal NgoMIV site found at 725
    Illegal NgoMIV site found at 872
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1004


Design Notes

Because there is a PstI cut site, so we process point mutation by over-lap PCR.synthetic biology proposes standard prefix and sufix, so we add prefix and sufix by PCR before we ligease it onto plasmid PSB1C3.


Source

It comes from Magnetospirillum magneticum AMB-1 bacteria strain.We firstly design primers to get this gene squence by PCR from genome, and then use another primer to add RBS J23106, prefix and sufix onto it.

References